Journal: iScience

Article Title: Distinct pathways utilized by METTL3 to regulate antiviral innate immune response

doi: 10.1016/j.isci.2024.111071

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-p-STAT2 , Cell Signaling Technology , Cat# 4441; RRID: AB_2198445.

Techniques: Virus, Recombinant, Transfection, Reverse Transcription, Enzyme-linked Immunosorbent Assay, Luciferase, SYBR Green Assay, Isolation, shRNA, Plasmid Preparation, Software

Journal: Oncology Letters

Article Title: Machine learning model reveals roles of interferon‑stimulated genes in sorafenib‑resistant liver cancer

doi: 10.3892/ol.2024.14571

Figure Lengend Snippet: Primers sequence used in SYBR-based reverse transcription-quantitative PCR.

Article Snippet: After blocking the membrane in TBS containing 5% skim milk for 1 h. The antibodies used for immunoblotting were as follows: rabbit monoclonal anti-STAT1 (Cell signaling Technology, Cat#9176S), rabbit monoclonal anti-PY STAT1 (Cell signaling Technology, Cat#9167S), rabbit polyclonal anti-STAT2 (Cell signaling Technology, Cat#4594S), rabbit polyclonal anti-PY STAT2 (Cell signaling Technology, Cat#4441S), rabbit monoclonal IRF9 (Cell signaling Technology, Cat#28492), and horseradish peroxidase-conjugated secondary antibody (1:5,000).

Techniques: Sequencing

Journal: Oncology Letters

Article Title: Machine learning model reveals roles of interferon‑stimulated genes in sorafenib‑resistant liver cancer

doi: 10.3892/ol.2024.14571

Figure Lengend Snippet: Increased IRF9 expression in sorafenib-resistant liver cancer cells. (A) Procedure for establishing sorafenib-resistant liver cancer cells. (B) liver cancer cells were treated with an increasing dose of sorafenib for 24 h. Cell viability was measured by MTT assay. (C) Protein levels of STAT1, STAT2 and IRF9 from immunoblotting. (D) mRNA levels of STAT1, STAT2 and IRF9 from reverse transcription-quantitative PCR. *P<0.05 and **P<0.01 vs. liver cancer cell lines (Huh-7 and HepG2). IRF9, interferon regulatory factor 9; wks, weeks; p-, phosphorylated.

Article Snippet: After blocking the membrane in TBS containing 5% skim milk for 1 h. The antibodies used for immunoblotting were as follows: rabbit monoclonal anti-STAT1 (Cell signaling Technology, Cat#9176S), rabbit monoclonal anti-PY STAT1 (Cell signaling Technology, Cat#9167S), rabbit polyclonal anti-STAT2 (Cell signaling Technology, Cat#4594S), rabbit polyclonal anti-PY STAT2 (Cell signaling Technology, Cat#4441S), rabbit monoclonal IRF9 (Cell signaling Technology, Cat#28492), and horseradish peroxidase-conjugated secondary antibody (1:5,000).

Techniques: Expressing, MTT Assay, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Machine learning model reveals roles of interferon‑stimulated genes in sorafenib‑resistant liver cancer

doi: 10.3892/ol.2024.14571

Figure Lengend Snippet: U-ISGs unresponsiveness depends on STAT1, STAT2 and IRF9 in Huh-7-SR cells. (A) Huh-7-SR cells were transfected with si-control, si-STAT1, si-STAT2, and si-IRF9. Then, 48 h after transfection, cells were harvested and immunoblotting of STAT1, STAT2 and IRF9 was performed. (B) mRNA levels of U-ISGs were measured by reverse transcription-quantitative PCR. (C) After transfection, Huh-7-SR cells were treated with an increasing dose of sorafenib for 24 h. **P<0.01 vs. siControl. IRF, interferon regulatory factor; si, small interfering; OAS1; oligoadenylate synthetase 1; IFI27, Interferon Alpha Inducible Protein 27.

Article Snippet: After blocking the membrane in TBS containing 5% skim milk for 1 h. The antibodies used for immunoblotting were as follows: rabbit monoclonal anti-STAT1 (Cell signaling Technology, Cat#9176S), rabbit monoclonal anti-PY STAT1 (Cell signaling Technology, Cat#9167S), rabbit polyclonal anti-STAT2 (Cell signaling Technology, Cat#4594S), rabbit polyclonal anti-PY STAT2 (Cell signaling Technology, Cat#4441S), rabbit monoclonal IRF9 (Cell signaling Technology, Cat#28492), and horseradish peroxidase-conjugated secondary antibody (1:5,000).

Techniques: Transfection, Control, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Machine learning model reveals roles of interferon‑stimulated genes in sorafenib‑resistant liver cancer

doi: 10.3892/ol.2024.14571

Figure Lengend Snippet: Mechanisms of U-ISGF3 complex in sorafenib resistance. U-ISGF complex, unphosphorylated interferon-stimulated gene factor-3; U-STAT1, unphosphorylated signal transducer and activator of transcription 1; U-STAT2, unphosphorylated signal transducer and activator of transcription 2; IRF9, interferon regulatory factor 9; U-ISGs, Unphosphorylated interferon-stimulated genes.

Article Snippet: After blocking the membrane in TBS containing 5% skim milk for 1 h. The antibodies used for immunoblotting were as follows: rabbit monoclonal anti-STAT1 (Cell signaling Technology, Cat#9176S), rabbit monoclonal anti-PY STAT1 (Cell signaling Technology, Cat#9167S), rabbit polyclonal anti-STAT2 (Cell signaling Technology, Cat#4594S), rabbit polyclonal anti-PY STAT2 (Cell signaling Technology, Cat#4441S), rabbit monoclonal IRF9 (Cell signaling Technology, Cat#28492), and horseradish peroxidase-conjugated secondary antibody (1:5,000).

Techniques: